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Wednesday, 1 August 2018

Standard E Dengue ELISA - Dengue NS1 Ag ELISA, Dengue IgM Capture ELISA and Dengue IgG Capture ELISA Key Features

Advantages of STANDARD E ELISA

    Standard E Dengue ELISA - Dengue NS1 Ag ELISA, Dengue IgM Capture ELISA and Dengue IgG Capture ELISA Key Features
  • Easy to use
  • Fast Results
  • Breakable Wells
  • Cost Effective

STANDARD E Dengue NS1 ELISA Kit

Dengue NS1 ELISA is a qualitative test for the detection of NS1 antigen from all serotypes (Den 1-4) of dengue virus in human serum or plasma. This test will help in the early detection of dengue virus even prior to seroconversion (presence of IgM or IgG antibodies).

Features of STANDARD E Dengue NS1 ELISA KIT

  • Qualitative detection of Dengue Ns1 antigen (Dengue Serotype 1,2,3 &4) in human serum or plasma.
  • Performance thoroughly evaluated with clinically confirmed cases of dengue 1-4 serotypes.
  • Employs a simple, one-step ratio method of interpretation.
  • Breakable wells to minimize wastage.
  • Assay Time- 1hr 40 minutes.

Test Procedure of Dengue  ELISA

Add 125 µl of positive (undiluted), negative controls (undiluted) & diluted samples in assigned wells.
                           ↓  Incubate at 37°C +/- 1°C for 1 hours
Wash 5 times with 350 µl prepared wash buffer giving 5 seconds soak time between each wash.
                           ↓
Add 100 of Enzyme Conjugate to each well.
                           ↓  Incubate at 37°C +/- 1°C for 1 hours
Wash 5 times with 350 prepared wash buffer giving 5 seconds soak time between each wash.
                          ↓
Add 100 of TMB Substrate to each well.
                          ↓     
Add 100 of Stop Solution to each well.
                          ↓
Read Result in ELISA Reader with primary wavelength 450 nm & secondary wavelength of 620-650 nm.

Performance characteristics Dengue NS1 ELISA

Sensitivity 98.18%
Specificity 95.38%

STANDARD E Dengue IgM Capture ELISA Kit

Dengue IgM capture ELISA is for the qualitative detection of IgM antibodies to Dengue virus recombinant antigens in human serum or plasma for the presumptive clinical laboratory diagnosis of Dengue virus infection. The assay is intended for use only in patients with clinical symptoms consistent with either dengue fever or dengue hemorrhagic fever. Use of Dengue IgM Capture ELISA simultaneously with Dengue IgG Capture ELISA will help in identifying primary & secondary infections of dengue.

Features of STANDARD E Dengue IgM Capture ELISA


Qualitative detection of IgM antibodies to Dengue antigen in human serum or plasma.
Performance thoroughly evaluated with clinically confirmed cases of dengue 1-4 serotypes.
Lyophilized Ag increases the stability & performance of the kit.
Employs a simple, one-step ratio method of interpretation.
No background color for clear result interpretation. 
Assay time -1 hour 40 minutes.

Performance characteristics Dengue IgM ELISA

Sensitivity 95.45%
Specificity 95.0%

STANDARD E Dengue IgG Capture ELISA Kit

Dengue IgG Capture ELISA test used for the Detection of  Dengue virus IgG antibodies in human serum or plasma to DenV recombinant antigen. This test is to aid in the diagnosis of human exposure to the Dengue virus. Use of Dengue IgG Capture ELISA simultaneously with Dengue IgM Capture ELISA will help in identifying & secondary infections of dengue.

Features of STANDARD E Dengue IgG Capture ELISA


Qualitative detection of IgG antibodies against dengue virus.
Helps identify past infections of dengue.
Lyophilized Ag enhances the stability and performance of the kit.
No background color for clear result interpretation.
Assay time 1 hour 40 minutes.

Performance characteristics Dengue IgM ELISA

Sensitivity 88.8%
Specificity 90.47%









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